Analyzer
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The 1st TiN Plasmonic
Lab-on-Chip Biosensor 1, 2
Quantitative real-time label-free biosensing of molecular interactions with
- Localized surface plasmon resonance (LSPR) of titanium nitride nanocubes;
- Massive parallel detection of over 144 channels per chip;
- Simplified functionalization chemistry;
- Optical measurement via interferometry;
- Friendly user interface;
- Automated functionalization (with robotic nanoliter dispenser).
Specifications3:
- 12 x 12 channels per chip with total target volume to 100 microliters
- < 100 nanoliters functionalization volume for each receptor species
- Biotinylation for receptor species before experiment
- Detection time is less than 15 minutes (Biotin-Streptavidin interaction)
Technical specifications and characteristics3
| Name | PLoC 122 System |
| Type | Label-free Analytical Biosensor |
| Detection technology | Localized Surface Plasmon Resonance Imaging (LSPRi) |
| Data presentation | Real-time sensorgrams of binding kinetics or statics of binding events |
| Analysis style | Microfludic array with optical phase measurement |
| Analysis time | 15 to 20 minutes per cycle |
| Number of flow cells | 12 x 12 = 144 cells in microfluidic array |
| Number of sensor spots | 1 to 144 (application-dependent) |
| Sample type | Small molecules to macromolecules in various fluidic conditions (e.g. heavy metal ions in water, exosome in serum, RNA in DEPC-treated water, etc.) |
| Sample volume | 100 uL |
| Injection volume | 360 to 720 uL |
| Flow rate range | 5 to 250 uL/min |
| Flow cell volume | 100 nL |
| Data acquisition rate |
1 Hz @ 4056 x 3040 x 32bits per pixel, 4 Hz @ 2028 x 1520 x 32bits per pixel |
| Sample capacity | 3 or 7 samples with automated degassing and injection |
| Typical run times |
20 minutes for 144 interactions or 1 hour for 432 interactions or 2.3 hours for 1008 interactions |
| Analysis temperature |
25 ℃ to 37 ℃ with standard option range, 25 ℃ to 75 ℃ with thermoplasmonic option |
| Sample storage |
4 ℃ for functionalised biochips Room temperature for bare biochips |
| Sample refractive index range |
1.33 to 1.47 |
| Real-time reference subtraction |
Automatic on demand |
| Dimensions |
350 x 550 x 190 mm |
| Net weight |
25 kg |
| Mains requirement |
100 to 240 V~, frequency 50/60 Hz |
| Power consumption |
max. 50 VA with standard option max. 100 VA with thermoplasmonic option |
| Probe laser |
Single mode 638 nm |
| Laser emission |
optical power 50 mW in test compartment optical power 0.01 mW with enclosure in operation |
|
Pump LED* (* for thermoplasmonic option only) |
Peak wavelength 590 nm |
| LED emission* |
optical power 1 W in test compartment optical power 0.01 mW with enclosure in operation |
Typical range of kinetic measurement3
| Association rate (ka) |
Macromolecule: up to 1 x 109 M-1 s-1 Small molecule: up to 1 x 107 M-1 s-1 |
| Dissociation rate (kd) | 10-6 to 10-1 s-1 |
| Sample concentration |
> 1 femtomolar (fM) for molecular weight less than 10 KDa; > 1 attomolar (aM) for molecular weight more than 100 KDa |
| Molecular weight | No lower limit for organic molecules |
| Short term noise |
typically 0.03 radian (σ) for standard edition |
| Baseline drift | typically 0.03 radian per hour |
| Functionalization | 1 nM to 1 uM of each 100 nL droplet |
Minimum computation requirements3
- Built-in CPU with 4 cores (standard edition)
- 8 GB internal memory
- 512GB solid-state hard drive space
- Screen resolution at least 1920x1080
- Gigabit Ethernet port for optional remote-data-analysis (RDA)
Remote data analysis (RDA) options3
- Ubuntu® LTS Server with CPU up to 64 cores
- 256 GB internal memory
- 28TB hard drive space
- Gigabit Ethernet port for data transfer from instrument
Demonstration of biosensing with Biotin-Streptavidin interaction:
References
1. Patent CN112033932B;
2. Patent CN112014924B;
3. Subject to improvement without notification
* CN115356300A, CN115290605A